s100b polyclonal antibody (Proteintech)

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Proteintech s100b polyclonal antibody
S100b Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slc7a11+polyclonal+antibody/SLC7A11%2FxCT+Antibody/pm41831507-42-16-28
Average 96 stars, based on 1123 article reviews

s100b polyclonal antibody - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA
Article Snippet: .. The primary antibodies used included GYS1 polyclonal antibody (1:2000, Cat. 10,566–1-AP, Proteintech), S100A12 polyclonal antibody (1:2000, Cat. 16,630–1-AP, Proteintech), and SLC7A11 polyclonal antibody (1:2000, Cat. 26,864–1-AP, Proteintech). (1:500–1:2000), incubated at 4°C overnight; the secondary antibody was HRP-labeled goat anti-rabbit IgG (1:15,000, GB23303, Seville Biotech) incubated at room temperature for 1 h, and the chemiluminescence method was used for imaging, with the gray value of the band analyzed using Image Lab software and normalized with the internal reference GAPDH. .. Statistical analysis was performed using GraphPad Prism 10.

Article Title: Artemisiae Scopariae Herba (Yinchen) suppresses ferroptosis in mice with osteoporosis via the Nrf2/Slc7a11/Gpx4 pathway
Article Snippet: Following dewaxing as aforementioned, the Prussian blue stain was prepared according to the manufacturer's instructions (Solarbio, G1422) and added at room temperature for 20 min, before sections were washed with distilled water for 5 min. .. Sections were incubated with the following primary antibodies: Anti-osteoprotegerin (OPG; 1:50; cat. no. ab73400; Abcam), anti-runt-related transcription factor 2 (RUNX2; 1:50; cat. no. ab236639; Abcam), Nrf2 polyclonal antibody (1:200; cat. no. 16396-1-AP; Proteintech Group, Inc.), Slc7a11 polyclonal antibody (1:50; cat. no. 26864-1-AP; Proteintech Group, Inc.) and Gpx4 monoclonal antibody (1:50; cat. no. 67763-1-Ig; Proteintech Group, Inc.) at 37°C overnight. .. Sections were incubated with an HRP-conjugated goat anti-rabbit IgG secondary antibody (1:2000; cat. no. GB23204; Wuhan Servicebio Technology Co., Ltd.) at 37°C for 120 min and biotinylated secondary antibodies (1:2,000; cat. no. PV-9001; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) for 120 min at room temperature.

Article Title: Integrating bioinformatic analyses and experimental validation of disulfidptosis-related genes and TMX4 in melanoma progression
Article Snippet: After transfer, the membrane was blocked using a rapid blocking solution (PS108P, Epizyme, China). .. The membrane was incubated with primary antibodies: TMX4 Polyclonal Antibody (21348-1-AP, Proteintech, USA; 1:1000), SLC7A11 Polyclonal Antibody (26864-1-AP, Proteintech, USA; 1:2000), and Beta Actin Monoclonal Antibody (66009-1-Ig, Proteintech, USA; 1:50000), followed by incubation with corresponding secondary antibodies: HRP-conjugated Goat Anti-Rabbit IgG (H + L) (SA00001-2, Proteintech, USA; 1:5000) and HRP-conjugated Goat Anti-Mouse IgG (SA00001-1-A, Proteintech, USA; 1:5000). .. Finally, the membrane was developed using enhanced chemiluminescent substrate (BL523B, Biosharp, China) and photographed.

Article Title: Integrating bioinformatic analyses and experimental validation of disulfidptosis-related genes and TMX4 in melanoma progression.
Article Snippet: After transfer, the membrane was blocked using a rapid blocking solution (PS108P, Epizyme, China). .. The membrane was incubated with primary antibodies: TMX4 Polyclonal Antibody (21348-1-AP, Proteintech, USA; 1:1000), SLC7A11 Polyclonal Antibody (26864-1-AP, Proteintech, USA; 1:2000), and Beta Actin Monoclonal Antibody (66009-1-Ig, Proteintech, USA; 1:50000), followed by incubation with corresponding secondary antibodies: HRP-conjugated Goat Anti-Rabbit IgG (H+L) (SA00001-2, Proteintech, USA; 1:5000) and HRP-conjugated Goat Anti-Mouse IgG (SA00001-1-A, Proteintech, USA; 1:5000). .. Finally, the membrane was developed using enhanced chemiluminescent substrate (BL523B, Biosharp, China) and photographed.

Article Title: Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA.
Article Snippet: .. The primary antibodies used included GYS1 polyclonal antibody (1:2000, Cat. 10566-1-AP, Proteintech), S100A12 polyclonal antibody (1:2000, Cat. 16630-1-AP, Proteintech), and SLC7A11 polyclonal antibody (1:2000, Cat. 26864-1-AP, Proteintech). (1:500-1:2000), incubated at 4°C overnight; the secondary antibody was HRP-labeled goat antirabbit IgG (1:15000, GB23303, Seville Biotech) incubated at room temperature for 1 hour, and the chemiluminescence method was used for imaging, with the gray value of the band analyzed using Image Lab software and normalized with the internal reference GAPDH. .. Statistical analysis was performed using GraphPad Prism 10.

Article Title: <p>Artemisiae Scopariae Herba (Yinchen) suppresses ferroptosis in mice with osteoporosis via the Nrf2/Slc7a11/Gpx4 pathway</p>.
Article Snippet: Sections were deparaffinized, antigen retrieval was performed using edTa antigen retrieval solution a and B (cat. no. SBT10013; Shunbai Biotechnology co., ltd.; both incubated at 37 ̊C for 30 min), and the sections blocked with 3% hydrogen peroxide at room temperature for 10 min for 30 min. Triton X‐100 (0.04%) was used to permeabilize the sections for 20 min at room temperature and goat serum (10%; cat. no. Zli‐9022; Beijing Zhongshan Jinqiao Biotechnology co., ltd.) was used to block the sections for 60 min at room temperature. .. Sections were incubated with the following primary antibodies: anti‐osteoprotegerin (oPG; 1:50; cat. no. ab73400; abcam), anti‐runt‐related transcription factor 2 (runX2; 1:50; cat. no. ab236639; abcam), nrf2 polyclonal antibody (1:200; cat. no. 16396‐1‐aP; Proteintech Group, inc.), Slc7a11 polyclonal antibody (1:50; cat. no. 26864‐1‐aP; Proteintech Group, inc.) and Gpx4 monoclonal antibody (1:50; cat. no. 67763‐1‐Ig; Proteintech Group, Inc.) at 37 ̊C over‐ night. .. Sections were incubated with an HrP‐conjugated goat anti‐rabbit igG secondary antibody (1:2000; cat. no. GB23204; Wuhan Servicebio Technology Co., Ltd.) at 37 ̊C for 120 min and biotinylated secondary antibodies (1:2,000; cat. no. PV‐9001; Beijing Zhongshan Jinqiao Biotechnology co., ltd.) for 120 min at room temperature.

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Article Title: Puerarin promotes nerve regeneration and functional recovery after peripheral nerve injury by inhibiting ACSL4-dependent ferroptosis.
Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 Peripheral nerve injury (PNI) is a common disease with poor treatment efficacy, it’s suggested that ferroptosis is closely related to the occurrence and development of PNI, but more in-depth studies are warranted.. Puerarin (Pu), an antioxidant from kudzu root, may mitigate PNI, but its mechanism remains unclear.. Here, we investigated Pu's role in PNI based on in vivo and in vitro experiments.

Colorimetric Assay:

Article Title: Magnetic traction and molecular lock-key: Nano-intelligent nanobot overcomes glioblastoma ferroptosis resistance to activate ferroptosis-immune
Article Snippet: RNA interference (RNAi) technology, which silences disease-causing genes, offers a novel therapeutic approach for glioblastoma (GBM).. However, the clinical translation of small interfering RNA (siRNA) faces challenges such as low delivery efficiency, rapid renal clearance, and endosomal/lysosomal entrapment.. This study developed a dual-targeted nanoplatform FZMT, enable precise siRNA delivery while synergistically inducing ferroptosis and antitumor immunity.

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